Part:BBa_K2074014

Extended FMDV 2A for Chlamydomonas reinhardtii

2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure7. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP

Fig.1 The Mechanism of 2A Peptide System

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 91
  
• 1000
  COMPATIBLE WITH RFC[1000]